Jordan Pickford was named England goalkeeper for the match and looks to be Southgate's first choice going into the World Cup, ahead of Jack Butland and Nick Pope.
The 21-year-old was involved in full training on Monday in their Austria camp and is expected to start against the Czechs in Vienna.
Nigeria's hurt may be enhanced given Alli's success as a growing footballer, signing for Spurs in 2015, and eventually earning his first England senior cap against Estonia in October 2016.
"But his decision making on crosses, the punch he made, his distribution and calmness to slide passes into midfield. that was really important to the way we want to play".
Chelsea defender Ola Aina and Bursaspor midfielder Mikel Agu will not be going to the 2018 World Cup in Russian Federation after Nigeria coach Gernot Rohr omitted them from his final 23-man squad for the tournament on Sunday.
Trippier claimed the assist for England's opening goal when his corner was headed home by Gary Cahill, and Southgate said: "His quality on the ball is outstanding". "We can not play like we did in the first half".
Their first World Cup group game is against Tunisia in Volgograd.
"I think the pace in the team, you can feel it, and that is why we had some problems in the first half".
"He has come out today in front of a huge crowd and performed very well".
13' CHANCE! England are running riots over their visitors and Raheem Sterling run over the Eagles defence to create two quick scoring opportunities.