Indonesian authorities have not raised the overall alert level, but did raise the Volcano Observatory Notice for Aviation, or VONA, from "orange" to "red", the top level.
The latest alert - a red warning - means an eruption is forecast to be "imminent" after nearby locals spotted volcanic ash rain.
Meanwhile, Garuda Indonesia flights with destinations from and to Bali to date continue to operate normally and smoothly, since to date flight routes from and to Bali are not affected by the volcanic ash spread from Mount Agung.
According to the Associated Press, the latest eruption on Mount Agung occurred around 5:30 p.m. Saturday, the National Disaster Mitigation Agency reported.
Mount Agung last erupted in 1963, killing over 1,600 people, and has been threatening another major eruption since August. Virgin, KLM and Air Asia Malaysia also cancelled several flights Saturday.
After Saturday's eruption, which produced a bigger ash cloud than the initial one on Tuesday, Singapore updated its travel advisory for the island, warning that ash clouds could "severely disrupt air travel".
Magma has been detected close to the surface on Mount Agung. Increased volcanic activity had prompted fears a major eruption was imminent.
Residents were evacuated from 224 points around the island while Lombok International Airport on Pulau Lombok, the island due east of Bali, has closed temporarily, said Ari Ahsan, spokesman for Ngurah Rai International Airport in Bali.
There have been no new evacuations in Bali and a six kilometre exclusion zone around the volcano has not been extended.
The Mt. Agung volcano, which towers over eastern Bali at a height of just over 3,000 meters (9,800 feet), is about 70 kilometers (43 miles) from the island's main tourist areas of Kuta and Seminyak. "Seismic activity is characterized by low frequency earthquakes".
About 25,000 people are thought to be in shelters while more than 140,000 people fled earlier this year.