The commission said that since 2008, Google has systematically given prominent placement to its own comparison shopping service, while demoting rival comparison shopping services in its search results.
A spokesman for Google confirmed the company had filed the appeal but said the company had no further comment to make. Google had initially said that they "respectfully" disagrees with the legal argument.
Google has chose to appeal the record-breaking fine imposed on it by the European Union's highest antitrust authority in July.
The fine on Google broke the previous record of European Union monopoly case against USA chip maker Intel of 1.06 billion euros in 2009.
For its part, Google was found guilty of misusing its search engine to promote another service, "Google Shopping", previously known as Google Product Search and Froogle, causing traffic drops of up to 90 percent for its competitors.
A court battle between Brussels and Google could take years to resolve and adds to an increasingly bitter row between the USA giant and European countries. It denied other companies the chance to compete on the merits and to innovate.
Regulators are also expected to levy fines in separate investigations into Google's Android mobile-phone software - possibly as soon as next month - and the AdSense advertising service.